Heme deficient Escherichia coli strain has a three-base-pair hotspot” in hemA by Authors Li J1, Deslouches B, Cosloy SD, Russell CS of City College of the City University of New York.
Heme-deficient Escherichia coli strain, Abstract
The key regulatory step in heme biosynthesis in Escherichia coli is at the level of glutamyl-tRNA reductase (GTR), an enzyme which is encoded by hemA. A strain, HU227, with a spontaneous in-frame mutation in hemA has no GTR activity. The mutation is shown to be a three-base deletion at a “hotspot” in the gene. The amino acid sequence in this region is highly conserved.
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